Immobilization of engineered arginase on gold-carbon nanotubes |
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Authors: | Gordon Kuen-Chan Lee Sui-Yi Kwok Chih Hao Yu Kin Tam Hiu-Chi Chong Yun-Chung Leung Shik Chi Edman Tsang |
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Institution: | Department of Chemistry, University of Oxford, Oxford, OX1 3QR, UK. |
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Abstract: | It is demonstrated that engineered arginase with a single protruded site of cysteine deliberately placed away from its active centre by site-directed mutagenesis can facilitate its attachment on a gold-nanoparticle surface with atomic precision, resulting in no apparent loss in enzymatic activity. |
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