Templated chemistry for monitoring damage and repair directly in duplex DNA |
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| Authors: | Seoung Ho Lee Shenliang Wang Eric T Kool |
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| Affiliation: | Department of Chemistry, Stanford University, Stanford, California 94305, USA. |
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| Abstract: | We report the fluorogenic detection of the product of base excision repair (an abasic site) in a specific sequence of duplex DNA. This is achieved by DNA-templated chemistry, employing triple helix-forming probes that contain unnatural nucleobases designed to selectively recognize the site of a missing base. Light-up signals of up to 36-fold were documented, and probes could be used to monitor enzymatic removal of a damaged base. |
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