Alternative Method for Forensic Determination of Lysergic Acid Diethylamide and Related Compounds in Body Fluids by Liquid–Liquid Extraction and HPLC with Fluorescence Detection |
| |
| Authors: | J Pietsch K Schulz B Körner H Trauer J Dreßler M Gey |
| |
| Institution: | (1) Institut für Rechtsmedizin, Medizinische Fakultät Carl Gustav Carus der TU Dresden, Fetscherstr. 74, 01307 Dresden, Germany;(2) Hochschule Zittau/Görlitz (FH), University of Applied Science, Fachbereich Mathematik/Naturwissenschaften, Fachgruppe Chemie, Külzufer 2, 02763 Zittau, Germany;(3) Institut für Rechtsmedizin, Medizinische Fakultät der Universität Leipzig, Johannisallee 28, 04103 Leipzig, Germany |
| |
| Abstract: | An alternative and practical method is described for simultaneous detection and quantification of the potent hallucinogen lysergic acid diethylamide (LSD) and related compounds in urine and serum samples. The procedure is based on liquid–liquid re-extraction with ethyl acetate and reversed-phase liquid chromatography coupled with fluorescence detection (HPLC–FLD). With detection limits in urine and serum samples of ca 0.07 ng mL–1 for LSD, nor-LSD, and iso-LSD, respectively, the method is well suited to forensic investigations. Application of the method to clinical samples and autopsy material enable selective identification and accurate quantification of LSD and related compounds. Comparison of results with those obtained from an LSD immunoassay (EMIT II) emphasize the need for chromatographic confirmation.Revised: 1 December 2003 and 9 February 2004 |
| |
| Keywords: | Column liquid chromatography Fluorescence detection Liquid– liquid re-extraction Immunoassay screening LSD nor-LSD and iso-LSD in urine and serum |
| 本文献已被 SpringerLink 等数据库收录！ |
|
