Improved mass accuracy in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of peptides |
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Authors: | Ole Vorm Matthias Mann |
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Affiliation: | 1. Department of Molecular Biology, Odense University, Odense, Denmark 2. Protein and Peptide Group, European Molecular Biology Laboratory (EMBL), Postfach 10.2209, D-69012, Heidelberg, Germany
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Abstract: | One problem of matrix-assisted laser desorption ionization coupled to time-of-flight mass spectrometry is the moderate mass accuracy that typically can be obtained in routine applications, Here we report improved mass accuracy for peptides, even when low amounts and complex peptide mixtures are used. A new procedure for preparing matrix surfaces is used, and there is no need to mix the matrix with the sample or to add internal standards. Examples are shown with a mass accuracy better than 50 ppm in a peptide mixture. Peptide mapping as well as sequencing by creating “ragged ends” or “ladder sequencing” should benefit especially from the improved mass accuracy. |
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