Calibration Point for Electron Ionization MS / MS spectra measured with multiquadrupole mass spectrometers |
| |
Authors: | Krishnan R. Mohan Michael G. Bartlett Kenneth L. Busch Alan E. Schoen Nigel Gore |
| |
Affiliation: | 1. School of Chemistry and Biochemistry, Georgia lnstitute of Technology, 30332-0400, Atlanta, GA, USA 2. Finnigan MAT, 355 River Oaks Parkway, San Jose, California, USA
|
| |
Abstract: | A protocol for establishing standard instrument conditions for measurement of product ion MS/MS spectra from parent ions produced by electron ionization is presented. Within this protocol, the ion at m/z 231 (C5F9 +) from perfluorokerosene or perfluorotributylamine is selected as the parent ion and subjected to collision-induced dissociation. The relative intensities of product ions at m/z 69, 131, and 181 are monitored as a function of collision energy while keeping the target gas pressure constant within the range of 10?4–10?6 torr (measured), or a beam attenuation of approximately 30-70%. The collision energy at which the ion intensities for product ions at m/z 69 and 181 are equal is defined as the calibration point at that collision gas pressure; the intensity of the ion at m/z 131 is very close to this value as well. Electron ionization MS/MS spectra taken at the calibration point using two different multiquadrupole instruments show good reproducibility for several test compounds. The high degree of similarity may aid in the establishment of a MS/MS spectral library. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|