Synthesis,characterization, DNA binding and nuclease activity of binuclear copper(II) complexes of cuminaldehyde thiosemicarbazones |
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Authors: | Panchangam Murali Krishna Katreddi Hussain Reddy Jay Prakash Pandey Dayananda Siddavattam |
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Institution: | (1) Department of Chemistry, Sri Krishnadevaraya University, Anantapur, 515 003, AP, India;(2) Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500 046, AP, India |
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Abstract: | Binuclear copper(II) complexes of thiosemicarbazones derived from cuminaldehyde (p-isopropyl benzaldehyde) and substituted thiosemicarbazides NH2NHC(S)NHR, where R = H, Me, Et or Ph have been synthesized and characterized. The ESR indicates that the dissociation of dimeric
complex into mononuclear Cu(L)Cl(DMSO)3] units in polar solvents like DMSO, where L = monoanionic thiosemicarbazone. The molecular ion peak in the LC-MS coincides
with the formula weight of the complexes. The absorption titration studies revealed that each of these complexes is an avid
binder to calf thymus-DNA. The apparent binding constants are in the order of 107–108 M−1. The nucleolytic cleavage activities of the ligands and their complexes were assayed on pUC18 plasmid DNA using gel electrophoresis
in the presence and absence of H2O2. The ligands showed increased nuclease activity when administered as copper complexes. All these copper(II) complexes behave
as an efficient chemical nucleases with hydrogen peroxide activation. These studies revealed that the complexes exhibit both
oxidative and hydrolytic chemistry in DNA cleavage. |
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