Use of SPE—TLC for Quality Control of Rhodiola rosea Extracts |
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Authors: | Kučinskaitė Agnė Pobłocka-Olech Loretta Krauze-Baranowska Mirosława Briedis Vitalis Savickas Aruñas Sznitowska Małgorzata |
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Affiliation: | 19.Department of Pharmaceutical Technology and Social Pharmacy, Kaunas Medical University, A. Mickeviciaus 9, 44307, Kaunas, Lithuania ;29.Department of Pharmacognosy, Medical University of Gdańsk, Hallera 107, 80-416, Gdańsk, Poland ;39.Department of Pharmaceutical Technology, Medical University of Gdańsk, Hallera 107, 80-416, Gdańsk, Poland ; |
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Abstract: | SPE and TLC have been used for qualitative and quantitative analysis of salidroside, rosavin, rosarin, and rosin in commercially available dry extracts from Rhodiola rosea roots. The best separation of all the compounds was achieved on silica gel TLC plates with ethyl acetate—methanol—water, 77 + 13 + 10 (v/v), as mobile phase. UV detection was performed at λ = 215 nm for salidroside and at λ= 245 nm for the rosavins (rosavin, rosarin, and rosin). Detection limits for salidroside and the rosavins were 90 ng and 60 ng per spot, respectively. Results from quantitative analysis confirmed the manufacturer’s declaration of the amounts of salidroside and the rosavins in the extracts. |
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