Dianthiamides A–E,Proline-Containing Orbitides from Dianthus chinensis |
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| Authors: | Jin Woo Lee Jun Gu Kim Jae Sang Han Yong Beom Cho Yu Jin Lee Dongho Lee Dae Hwan Shin Jin Tae Hong Mi Kyeong Lee Bang Yeon Hwang |
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| Institution: | 1.College of Pharmacy, Chungbuk National University, Cheongju 28160, Korea; (J.W.L.); (J.G.K.); (J.S.H.); (Y.B.C.); (Y.J.L.); (D.H.S.); (J.T.H.); (M.K.L.);2.Department of Plant Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea; |
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| Abstract: | Orbitides are plant-derived small cyclic peptides with a wide range of biological activities. Phytochemical investigation of the whole plants of Dianthus chinensis was performed with the aim to discover new bioactive orbitides. Five undescribed proline-containing orbitides, dianthiamides A–E (1–5), were isolated from a methanolic extract of Dianthus chinensis. Their structures were elucidated by extensive analysis of 1D and 2D NMR and HRESI–TOF–MS as well as ESI–MS/MS fragmentation data. The absolute configuration of the amino acid residues of compounds 1–5 was determined by Marfey’s method. All compounds were tested for their cytotoxic activity, and dianthiamide A (1) exhibited weak activity against A549 cell line with IC50 value of 47.9 μM. |
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| Keywords: | Dianthus chinensis Caryophyllaceae orbitide structure elucidation cytotoxic activity |
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