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Duplex Surface Enhanced Raman Scattering-Based Lateral Flow Immunosensor for the Low-Level Detection of Antibiotic Residues in Milk
Authors:Ruiqi Fan  Shusheng Tang  Sunlin Luo  Hu Liu  Wanjun Zhang  Chunjiang Yang  Lidong He  Yiqiang Chen
Affiliation:1.State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; (R.F.); (S.L.); (H.L.); (W.Z.);2.College of Veterinary Science, China Agricultural University, Beijing 100193, China; ;3.Ring Biotechnology Co Ltd., Building 7, Bodaxing Industry Park, BDA, Beijing 101111, China; ;4.Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA;
Abstract:A duplex surface enhanced Raman scattering (SERS)-based lateral flow immunosensor was established for the simultaneous detection of two common antibiotic residues including tetracycline and penicillin in milk. The newly synthesized Au@Ag nanoparticles were labeled with different Raman molecules including 5,5-dithiobis-2-nitrobenzoic acid (DTNB) or 4-mercaptobenzoic acid (MBA), followed by the conjugation of anti-tetracycline monoclonal antibody or anti-penicillin receptor, forming two kinds of SERS nanoprobes. The two nanoprobes can recognize tetracycline-BSA and ampicillin-BSA, respectively, which facilitates the simultaneous detection of the two types of antibiotics on a single test line. After optimization, detection limits of tetracycline and penicillin as low as 0.015 ng/mL and 0.010 ng/mL, respectively, were achieved. These values were far below those of most of other documented bio-analytical approaches. Moreover, the spiking test demonstrates an excellent assay accuracy with recoveries of 88.8% to 111.3%, and satisfactory assay precision with relative standard deviation below 16%. Consequently, the results demonstrate that the SERS-based lateral flow immunosensor developed in this study has the advantages of excellent assay sensitivity and remarkable multiplexing capability, thus it will have great application potential in food safety monitoring.
Keywords:surface enhanced raman scattering   immunosensor   tetracycline   penicillin   milk
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