Duplex Surface Enhanced Raman Scattering-Based Lateral Flow Immunosensor for the Low-Level Detection of Antibiotic Residues in Milk |
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Authors: | Ruiqi Fan Shusheng Tang Sunlin Luo Hu Liu Wanjun Zhang Chunjiang Yang Lidong He Yiqiang Chen |
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Affiliation: | 1.State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; (R.F.); (S.L.); (H.L.); (W.Z.);2.College of Veterinary Science, China Agricultural University, Beijing 100193, China; ;3.Ring Biotechnology Co Ltd., Building 7, Bodaxing Industry Park, BDA, Beijing 101111, China; ;4.Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA; |
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Abstract: | A duplex surface enhanced Raman scattering (SERS)-based lateral flow immunosensor was established for the simultaneous detection of two common antibiotic residues including tetracycline and penicillin in milk. The newly synthesized Au@Ag nanoparticles were labeled with different Raman molecules including 5,5-dithiobis-2-nitrobenzoic acid (DTNB) or 4-mercaptobenzoic acid (MBA), followed by the conjugation of anti-tetracycline monoclonal antibody or anti-penicillin receptor, forming two kinds of SERS nanoprobes. The two nanoprobes can recognize tetracycline-BSA and ampicillin-BSA, respectively, which facilitates the simultaneous detection of the two types of antibiotics on a single test line. After optimization, detection limits of tetracycline and penicillin as low as 0.015 ng/mL and 0.010 ng/mL, respectively, were achieved. These values were far below those of most of other documented bio-analytical approaches. Moreover, the spiking test demonstrates an excellent assay accuracy with recoveries of 88.8% to 111.3%, and satisfactory assay precision with relative standard deviation below 16%. Consequently, the results demonstrate that the SERS-based lateral flow immunosensor developed in this study has the advantages of excellent assay sensitivity and remarkable multiplexing capability, thus it will have great application potential in food safety monitoring. |
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Keywords: | surface enhanced raman scattering immunosensor tetracycline penicillin milk |
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