STUDY ON THERMAL DENATURATION OF PROTEINS CONTAINING LOW WATER CONTENT BY DIFFERENTIAL SCANNING CALORIMETER——DEMONSTRATION OF PRE-THERMODENATURE NEW PEAK

Following the discovery that there is a low temperature endothermal peak for hydration bovine serum albumin, this work further measured DSC heating scanning curve for seven different kinds of hydration proteins with high purity from eleven sources. The measurements have shown that there is an obvious low temperature endothermal peak for all of the protein samples used. We term it "Pre-thermodenature New Peak". The appearance of pre-thermodenature new peak is the common property of proteins containing low water content. The studies of regularity and mechanism on new peak should help to understand the denaturation process of protein, protein folding, molecular association and/or molecular disassociation and solvent effect.