DNA cleavage and antimicrobial studies of 17-membered schiff base macrocyclic triazoles: synthesis and spectroscopic approach

A novel series of 17-membered complexes MLCl₂] (M = Co²⁺, Ni²⁺ and Cu²⁺) have been synthesized with newly derived biologically active ligands (L^I–L^IV). These ligands were synthesized by the condensation of 3-subtituted-4-amino-5-hydrazino-1,2,4-triazole with bis(phthalaldehyde)ethylenediamine precursor. The structure of the complexes has been proposed by elemental analyses, IR, EPR, electronic spectral studies, conductivity, magnetic, thermal and electrochemical studies. All the complexes are soluble in DMF and DMSO and are non-electrolytes. All these Schiff bases and their complexes have been screened for their antibacterial (Escherichia coli, Staphylococus aureus, Salmonella typhi, Pseudomonas aeruginosa) and antifungal activities (Aspergillus niger, Aspergillus flavus and Cladosporium) by the Agar and Potato dextrose agar diffusion method. The DNA cleavage study was done by Agarose gel electrophoresis technique.