Separation of salidroside from Rhodiola crenulata by high-speed counter-current chromatography |
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Authors: | Han Xiao Zhang Tianyou Wei Yun Cao Xueli Ito Yoichiro |
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Affiliation: | Beijing Research Center for Separation & Purification Technologies of Natural Products, Beijing Institute of New Technology Application, China. |
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Abstract: | High-speed counter-current chromatography (HSCCC) was used to purify salidroside from an extract of Rhodiola crenulata with two steps using a two-phase solvent system composed of ethyl acetate-n-butanol-water (1:4:5, v/v) in the first run and chloroform-methanol-isopropanol-water (5:6:1:4) in the second run. The method yielded 21.9 mg of salidroside from 1.216 g of the crude sample at 98% purity determined by HPLC analyses. Identification was performed by 1H NMR, 13C NMR, and MS. |
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