Rapid RP-LC Method with Fluorescence Detection for Analysis of Fexofenadine in Human Plasma |
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Authors: | Lucyna Konieczna Alina Plenis Ilona Olędzka Piotr Kowalski Tomasz Bączek |
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Institution: | 1. Department of Pharmaceutical Chemistry, Medical University of Gdańsk, 80-416, Gdańsk, Poland
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Abstract: | A rapid and sensitive reversed-phase high-performance liquid chromatographic method for analysis of fexofenadine in human plasma has been developed and optimized. The analytes were extracted from biological samples by solid-phase extraction on hydrophilic–lipophilic balance cartridges. LC separation was performed on a C18 analytical column (125 mm × 4 mm i.d., 5-μm particles) with 42:58 (v/v) acetonitrile–water adjusted to pH 2.7 with 85% orthophosphoric acid as mobile phase. Fluorescence detection was performed with excitation at 230 nm and emission at 290 nm. The total time for chromatographic separation was 7 min. The method was validated in accordance with EU guidelines by analysis of plasma samples fortified with fexofenadine at concentrations between 0.05 and 800 ng mL?1. Calibration plots were linear in this range. Mean recovery was typically 94.03% and the detection limit was 0.05 ng mL?1. The time required for quantitative analysis is shorter than that required by other methods. |
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