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A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles |
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| Authors: | Rosa Pereira Tommy Julianto Peh-Kim Ang Sharon Sheue-Nee Ling Carlos Mauricio Barbosa Kah-Hay Yuen Abu Bakar Abdul Majeed |
| Affiliation: | 1. Nanopharmacy Research Unit, Faculty of Pharmacy, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia 2. School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pinang, Malaysia 3. Hovid Bhd, 121, Jalan Tunku Abdul Rahman, Ipoh, Perak, Malaysia 4. Servi?o de Tecnologia Farmacêutica, Faculdade de Farmácia da Universidade do Porto and CEQUIMED-UP, Centro de Química Medicinal da Universidade do Porto, Porto, Portugal |
| Abstract: | A sensitive and rapid routine LC method was validated for measuring cefotaxime incorporated in three different pH-sensitive nanoparticles. The drug was chromatographed on a C18 reversed-phase column; the mobile phase used was 0.05 M aqueous ammonium acetate, acetonitrile and tetrahydrofuran (87:11:2, v/v) adjusted to pH 5.5 with acetic acid. The flow rate was 1 mL min?1 and cefotaxime was quantified at 254 nm, with a sensitivity range of 0.005 AUFS. The validated method was specific, linear (R 2 ≥ 0.999), precise and accurate in a concentration range of 0.2–50.0 μg mL?1. The method was rapid, selective and suitable for evaluation of cefotaxime in pH-sensitive Eudragit nanoparticles. |
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