Selectivity Differences Between ODS and Polar Modified Stationary Phases in RP-LC |
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Authors: | Zhuo Xiang Xiaoli Sun Peng Liu Xiaoye Li Ru Jiang |
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Institution: | 1. Department of Chemistry, School of Pharmacy, Fourth Military Medical University, Xi’an, 710032, China 2. Department of Medicinal Chemistry and Pharmaceutical Analysis, School of Pharmacy, Fourth Military Medical University, Xi’an, 710032, China
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Abstract: | Several new octadecylsilyl and polar-modified phases were synthesized. Chromatographic evaluations of these columns were performed using the Dolan and Engelhardt test mixtures. The applicability of these new phases was also evaluated with a mixture of acids, bases or neutral compounds. The distinct differences in the chromatographic behavior between the two groups as well as a high degree of variability within each group were observed. The polar-endcapped phases exhibit similar hydrophobic selectivity, higher hydrogen bonding capacities and silanol activity with reference to C18 columns. The polar-embedded phases display a greatly reduced hydrophobic nature and a significantly reduced silanol activity compared to the C18 phases. |
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