Rapid Determination of Water-Soluble and Fat-Soluble Vitamins in Commercial Formulations by MEEKC |
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Authors: | María S Aurora-Prado Claudinei A Silva Marina F M Tavares Kevin D Altria |
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Institution: | 1. Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 580, Sao Paulo, SP, 05508-000, Brazil 2. Institute of Chemistry, University of Sao Paulo, Av. Prof. Lineu Prestes, 748, Sao Paulo, SP, 05508-000, Brazil 3. GlaxoSmithKline Pharmaceutical Development, Harlow, Essex, CM19 5AW, UK
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Abstract: | Microemulsion electrokinetic capillary chromatography has been successfully applied to the separation and determination of water-soluble vitamins (thiamine hydrochloride, riboflavin, niacin, pyridoxine hydrochloride, folic acid, cobalamin, ascorbic acid) and a fat-soluble vitamin (α-tocopherol acetate). The optimal microemulsion buffer contained sodium dodecylsulfate (SDS) as surfactant, butan-1-ol as the co-surfactant, ethyl acetate as the oil and pH 9.2 tetraborate buffer, modified with 15% (v/v) 2-propanol. UV detection at 214 nm gave adequate sensitivity without interference from sample excipients. Under the optimized conditions, the vitamins were baseline separated in less than 7 min. Analytical curves of peak area versus concentration presented coefficients of determination (R 2 ) > 0.99, acceptable limits of quantification between 8.40 and 16.23 μg mL?1 were obtained. Vitamin levels in liquid formulation were quantified with intra-day precision better than 0.99% RSD for migration time and 1.19% RSD for peak area ratio. Recoveries ranged between 98.7 and 101.7%. The method was considered appropriate for rapid and routine analysis. |
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