Preparative Isolation and Purification of Two Phenylpropanoids from Daphne giraldii Nitsche by HSCCC |
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Authors: | Feng-Yun Li Zhan-Ping Gou Xiao-Chi Ma Yan Tian Ge Tian Chang-Yuan Wang Dong-Hai Su Ke Xin Liu Xiu-Lan Xin |
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Institution: | 1. School of Pharmacy, Dalian Medical University, 116044, Dalian, People’s Republic of China 2. School of Pharmacology, Guangdong Medical College, 523808, Dongguan, China 3. Biotechnology Application Center, Beijing Vocational College of Electronic Science and Technology, Beijing, China
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Abstract: | Preparative high-speed counter-current chromatography (HSCCC) was successfully applied to purify phenylpropanoids from the stem and root bark of Daphne giraldii Nitsche, a traditional Chinese medicine. Their structures were identified on the basis of 1H NMR and 13C NMR technology. The two-phase solvent system composed of n -hexane–ethyl acetate–methanol–water (2: 3: 0.5: 4, v/v/v/v) was selected for HSCCC. A total of 8.0 mg woonenoside XI (1) and 18.0 mg daphnetin (2) were obtained in one-step separation from 200 mg of the crude extract with purity of 96.0 and 99.1%, respectively, as determined by LC. And the major compound (2) showed antithrombotic activity in vitro. |
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