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Towards the biotechnological isomerization of branched sugars: d-tagatose-3-epimerase equilibrates both enantiomers of 4-C-methyl-ribulose with both enantiomers of 4-C-methyl-xylulose |
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| Authors: | Devendar Rao Pushpakiran Gullapalli Goro Takata Sarah F Jenkinson Raymond A Dwek Ken Izumori |
| Institution: | a Rare Sugar Research Center, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Mik-choi, Kita-gun, Kagawa 761-0795, Japan b Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Mansfield Road OX1 3TA, UK c Glycobiology Institute, Department of Biochemistry, Oxford University, South Parks Road, Oxford OX1 3QU, UK |
| Abstract: | Microbial oxidation of 2-C-methyl-d-ribitol and 2-C-methyl-d-arabinitol by Gluconobacter thailandicus NBRC 3254 produces 4-C-methyl-l-ribulose and 4-C-methyl-d-ribulose, respectively. Further, 4-C-methyl-l-ribulose and 4-C-methyl-d-ribulose were equilibrated by d-tagatose-3-epimerase (DTE) with 4-C-methyl-l-xylulose and 4-C-methyl-d-xylulose, respectively. These transformations demonstrate that polyol dehydrogenase and DTE act on branched synthetic sugars. The green preparation of all of the stereoisomers of 4-C-methyl pentuloses illustrates the ability of biotechnology to generate novel branched monosaccharides. |
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