Synthesis of Nitroanilines Catalyzed by Horseradish Peroxidase in the Presence of NaNO2 and H2O2

The synthesis of nitroanilines catalyzed by horseradish peroxidase(HRP) in the presence of sodium nitrite and hydrogen peroxide was investigated. o-Nitroaniline and p-nitroaniline were found in the nitrated products. 2-Aminotoluene and 4-aminotoluene could also be nitrated to give corresponding nitrated products. This protocol has great potentials to open new avenues useful for the synthesis of nitroaniline and its derivatives.