Development and characterization of an integrated silicon micro flow cytometer |
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Authors: | R Bernini E De Nuccio F Brescia A Minardo L Zeni P M Sarro R Palumbo M R Scarfi |
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Institution: | (1) IREA-CNR, Via Diocleziano 328, 80124 Napoli, Italy;(2) DII, Seconda Università di Napoli, Via Roma 29, 81031 Aversa, Italy;(3) ECTM-DIMES, TUDelft, Feldmannweg 17, 2600 Delft, The Netherlands;(4) IBB-CNR, Via Mezzocannone 16, 80134 Napoli, Italy |
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Abstract: | This paper describes an innovative integrated micro flow cytometer that presents a new arrangement for the excitation/detection
system. The sample liquid, containing the fluorescent marked particles/cells under analysis, is hydrodynamically squeezed
into a narrow stream by two sheath flows so that the particles/cells flow individually through a detection region. The detection
of the particles/cells emitted fluorescence is carried out by using a collection fiber placed orthogonally to the flow. The
device is based on silicon hollow core antiresonant reflecting optical waveguides (ARROWs). ARROW geometry allows one to use
the same channel to guide both the sample stream and the fluorescence excitation light, leading to a simplification of the
optical configuration and to an increase of the signal-to-noise ratio. The integrated micro flow cytometer has been characterized
by using biological samples marked with standard fluorochromes. The experimental investigation confirms the success of the
proposed microdevice in the detection of cells.
An erratum to this article can be found at |
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Keywords: | Microfluidics Microfabrication Optical sensors Biochips High-throughput screening |
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