| 反相高效液相色谱分离果酸的研究 |
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| 引用本文： | 倪尔葭,黄晓兰,谢曼丹.反相高效液相色谱分离果酸的研究[J].分析测试学报,1991(3). |
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| 作者姓名： | 倪尔葭 黄晓兰 谢曼丹 |
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| 作者单位： | 广州化学研究所
(倪尔葭，黄晓兰)，广州化学研究所(谢曼丹) |
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| 摘 要： | 水果和蔬菜中含有的有机酸,称谓果酸,果酸的定量方法很多,气相色谱法需要将果酸衍生后才能分析。本文阐述用反相高效液相色谱及紫外与示差折光检测器,流动相为0.01M(NH_4)_2HPO_4,用H_3PO_4调节pH值为2.5,使果酸得到分离。用保留时间定性,用标准酸样的直线方程进行定量。并对橙子中的柠檬酸、健力宝饮料中的酒石酸作了定量测定。回收率达90%以上。此法快速,分离效果较好,是食品生产与分析发展的趋势。
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| 关 键 词： | 果酸 高压液相色谱 分离 保留时间 |
Study on the Separation of Carboxylic Acids in Fruits by RPLC |
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| Ni Erjia,Huang Xiaolan,Xie Mandan Guangzhou Institute of Chemistry,Academia Sinica.Study on the Separation of Carboxylic Acids in Fruits by RPLC[J].Journal of Instrumental Analysis,1991(3). |
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| Authors: | Ni Erjia Huang Xiaolan Xie Mandan Guangzhou Institute of Chemistry Academia Sinica |
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| Abstract: | A series of carboxylic acids in fruits has been separated simultaneously by RPLC withultraviolet of 214nm and refractive index detector and a mobile phase of pH 2.5. The acids thusseparated could be identified by their retention times, and the quantity of those acids could bedetermined by calibration curve comparation method. Citric acid in oranges and tartaric acid in abeverage have been determined by this method with a recovery of above 90%. |
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| Keywords: | Carboxylic acids RPLC Separation retention time |
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