Interrogation of living cells using alternating current scanning electrochemical microscopy (AC-SECM) |
| |
Authors: | Diakowski Piotr M Ding Zhifeng |
| |
Institution: | Department of Chemistry, The University of Western Ontario, London, ON, Canada. |
| |
Abstract: | In this paper we present the application of alternating current scanning electrochemical microscopy (AC-SECM) to the study of living cells. Commercial AFM instrumentation was modified to allow for performing robust AC-SECM measurements. Constant height AC imaging of the Cos-7 cells, performed directly in cell culture medium without the addition of a redox mediator, provided topographical information of the cell. Stationary tip measurements on the AC current were carried out to investigate the cellular activity of a single cell. The dependence of AC current magnitude on tip-to-sample separation distance was used to monitor real time changes in cell height of individual Cos-7 cells. Furthermore, AC-SECM was employed to observe changes in metabolic cellular activity stimulated by ethanol and phorbol-1,2-myristate-acetate-3. The effect of changing cellular activity on constant height AC-SECM imaging was also studied. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|