| Crystal Structure of Crotin Ⅱ at 2.5 Resolution |
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| 作者姓名： | ZHOU Kang-Jing FU Zhu-Ji CHEN Ming-Huang QIU Wei PAN Ke-Zhen |
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| 作者单位： | Fujian Institute of Research on the Structure of Matter，the Chinese Academy of Sciences，Fuzhou，Fujian 350002 ChinaNational Laboratory of Biomacromolecule，Beijing 100101，China |
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| 摘 要： | 1INTRODUCTIONTheplanttoxin,CrotinIisoneoftheribosomeinactivatingproteins(RIPs)l3.AccordingtothemolecularmechanismofactiontheRIPscanbedividedintotwokinds2:.OnebelongstoRNAN-glycosidasewhichisclassifiedastwotypes.TypeIconsistsofasinglepolypeptidechain,forexample,trichosanthinandsaporin-Typellconsistsoftwochains,suchasricinandabrin'ThiskindofRIPshydrolysesasingleN-glycosidicbondbetweenanadenineandriboseatspecificnu-cleotide(A4324)of28SrRNAintheribosomes3i.ThesecondkindofRIPsbel…
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| 关 键 词： | crotin Ⅱ crystal structure molecular replacement method |
Crystal Structure of Crotin Ⅱ at 2. 5 Resolution |
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| ZHOU Kang-Jing, FU Zhu-Ji, CHEN Ming-Huang ,QIU Wei, PAN Ke-Zhen.Crystal Structure of Crotin Ⅱ at 2. 5 Resolution[J].Chinese Journal of Structural Chemistry,1997(3). |
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| Authors: | ZHOU Kang-Jing FU Zhu-Ji CHEN Ming-Huang QIU Wei PAN Ke-Zhen |
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| Abstract: | Crotin Ⅱ is one of the ribosome inactivating proteins (RIPs). It belongs to RNA hydrolase. The crystals of crotin Ⅱ are hexagonal with a=b= 94. 62, c=28. 44 A, space group P61, Mr= 14900 Da. The structure was solved by molecularreplacement methed using the molecular structure of RNase T1 as a search model andrefined to R= 0. 25 for the reflections within 10- 2. 5 A resolution range. The refinedmodel cotains one α-helix, one two-strand antiparallel β-sheet and one five-strand an-tiparallel β-sheet. Four conservative residues His47, Glu77, Arg102 and His117 gathering in the cleft of the structure form the possible active site of crotin Ⅱ. |
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| Keywords: | crotin Ⅱ crystal structure molecular replacement method |
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