| 三聚氰胺对DNA潜在损伤作用的研究 |
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| 引用本文： | 张国文,汪佳蓉,杨佳. 三聚氰胺对DNA潜在损伤作用的研究[J]. 分析试验室, 2010, 29(2) |
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| 作者姓名： | 张国文 汪佳蓉 杨佳 |
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| 作者单位： | 南昌大学食品科学与技术国家重点实验室,南昌,330047;南昌大学食品科学与技术国家重点实验室,南昌,330047;南昌大学食品科学与技术国家重点实验室,南昌,330047 |
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| 基金项目： | 科技部南昌大学国家重点实验室基金(SKLF-MB-200807);;江西省自然科学基金(2007GZH1924)项目资助 |
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| 摘 要： | 在生理酸度条件下(pH 7.4),采用溴化乙锭(EB)为荧光探针的荧光光谱法、I-离子荧光猝灭效应、DNA熔点和粘度效应等手段,研究了三聚氰胺与DNA的相互作用。随着DNA的加入,三聚氰胺的荧光强度明显减小而且三聚氰胺能够猝灭DNA-EB复合物的荧光,说明三聚氰胺能够竞争置换EB而与DNA作用;三聚氰胺的加入使得DNA的粘度增大,DNA-EB的熔点降低;DNA的加入减小了I-对三聚氰胺荧光的猝灭程度。三聚氰胺以嵌插方式作用于DNA的亲核位点,意味着三聚氰胺进入生物体后有可能通过形成DNA加合物的形式造成DNA损伤,从而最终导致基因突变。
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| 关 键 词： | 三聚氰胺 DNA 荧光光谱 DNA加合物 |
Study of influences of melamine on potential trauma of DNA |
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| ZHANG Guo-wen,WANG Jia-rong,YANG Jia. Study of influences of melamine on potential trauma of DNA[J]. Chinese Journal of Analysis Laboratory, 2010, 29(2) |
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| Authors: | ZHANG Guo-wen WANG Jia-rong YANG Jia |
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| Affiliation: | State Key Laboratory of Food Science and Technology;Nanchang University;Nanchang 330047 |
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| Abstract: | The interaction between melamine and calf thymus DNA in physiological buffer(pH 7.4) was explored by using ethidium bromide(EB) as a fluorescence probe.The experimental phenomena showed that fluorescence intensity of melamine was greatly reduced with the addition of DNA.Moreover,fluorescence quenching of the emission peak was observed in the DNA-EB system when melamine was added,suggesting that a strong competition for DNA binding between melamine and EB.The relative viscosity of DNA increased with the addi... |
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| Keywords: | Melamine DNA Fluorescence spectroscopy DNA adduct |
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