Structure of octaheme cytochrome <Emphasis Type="Italic">c</Emphasis> nitrite reductase from <Emphasis Type="Italic">Thioalkalivibrio nitratireducens</Emphasis> in a complex with phosphate |
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Authors: | A A Trofimov K M Polyakov K M Boĭko A A Filimonenkov P V Dorovatovskiĭ T V Tikhonova V O Popov M V Koval’chuk |
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Institution: | 1.Engelhardt Institute of Molecular Biology,Russian Academy of Sciences,Moscow,Russia;2.Bach Institute of Biochemistry,Russian Academy of Sciences,Moscow,Russia;3.Kurchatov Center for Synchrotron Radiation and Nanotechnology,Moscow,Russia;4.Shubnikov Institute of Crystallography,Russian Academy of Sciences,Moscow,Russia |
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Abstract: | Octaheme cytochrome c nitrite reductase from Thioalkalivibrio nitratireducens (TvNiR) catalyzes the reduction of nitrite and hydroxylamine to ammonia. The structures of the free enzyme and of the enzyme in complexes with the substrate (nitrite ion) and the inhibitor (azide ion) have been solved previously. In this study we report the structures of the oxidized complex of TvNiR with phosphate and of this complex reduced by europium(II) chloride (1.8- and 2.0-Å resolution, the R factors are 15.9 and 16.7%, respectively) and the structure of the enzyme in the complex with cyanide (1.76-Å resolution, the R factor is 16.5%), which was prepared by soaking a crystal of the oxidized phosphate complex of TvNiR. In the active site of the enzyme, the phosphate ion binds to the iron ion of the catalytic heme and to the side chains of the catalytic residues Arg131, Tyr303, and His361. The cyanide ion is coordinated to the heme-iron ion and is hydrogen bonded to the residue His361. In the structure of reduced TvNiR, the phosphate ion is bound in the same manner as in the structure of oxidized TvNiR, and the nine_coordinated europium ion is located on the surface of one of the crystallographically independent monomers of the enzyme. |
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