Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source |
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| Authors: | Owen Dylan M Auksorius Egidijus Manning Hugh B Talbot Clifford B de Beule Pieter A A Dunsby Christopher Neil Mark A A French Paul M W |
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| Institution: | Chemical Biology Centre, Photonics Group, Department of Physics, Imperial College London, UK. dylan.owen00@imperial.ac.uk |
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| Abstract: | We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image. |
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