In situ data collection and structure refinement from microcapillary protein crystallization |
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Authors: | Yadav Maneesh K Gerdts Cory J Sanishvili Ruslan Smith Ward W Roach L Spencer Ismagilov Rustem F Kuhn Peter Stevens Raymond C |
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Affiliation: | The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. |
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Abstract: | In situ X-ray data collection has the potential to eliminate the challenging task of mounting and cryocooling often fragile protein crystals, reducing a major bottleneck in the structure determination process. An apparatus used to grow protein crystals in capillaries and to compare the background X-ray scattering of the components, including thin-walled glass capillaries against Teflon, and various fluorocarbon oils against each other, is described. Using thaumatin as a test case at 1.8 ? resolution, this study demonstrates that high-resolution electron density maps and refined models can be obtained from in situ diffraction of crystals grown in microcapillaries. |
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