Cryopreservation of cultivated and wild Arachis species embryonic axes using desiccation and vitrification methods |
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| Authors: | Gagliardi R F Pacheco G P Valls J F M Mansur E |
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| Institution: | Laboratório de Micropropaga??o e Transforma??o de Plantas, Universidade do Estado do Rio de Janeiro, Brazil. |
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| Abstract: | The effects of two methods of cryopreservation involving chemical vitrification and air desiccation) were studied on isolated embryonic axes of A. hypogaea. Vitrification with PVS2 and desiccation in a laminar flow cabinet resulted in high levels (70-90%) of whole plant recovery after cryopreservation. A desiccation protocol based on 1h exposure of explants to the air flow was successfully applied to six wild species of section Extranervosae, resulting in recovery levels of 70-90% after liquid nitrogen treatment. |
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