Spectrophotometric determination of serum iron with a new reagent |
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| Authors: | H. Alexaki-Tzivanidou |
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| Affiliation: | Laboratory of Physiology, Veterinary Faculty, University of Thessaloniki, Thessaloniki, Greece |
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| Abstract: | The use of 2,2′-dipyridyl-2-pyridylhydrazone (DPPH) as a new “ferroin” type color reagent for the spectrophotometric determination of serum iron is presented. The determination is performed on protein-free filtrates at pH 1.6 to 3.0. The reagent has been found to be specific for serum iron, under the proposed conditions. The color of the water-soluble iron-DPPH complex is considerably stable. |
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