Determination of bile acids in serum and bile by a modified gas chromatographic glass capillary technique |
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| Authors: | W. Swobodnik W. Nebelung U. Klueppelberg J. G. Wechsler P. Janowitz H. Wenk H. Ditschuneit |
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| Affiliation: | (1) Department of Internal Medicine II, Gastroenterology and Nutrition, University Clinic Ulm, D-7900 Ulm, FRG |
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| Abstract: | Summary A modified gas chromatographic glass capillary technique for determination of five major bile acids (Cholic acid: CA, Chenodeoxycholic acid: CDCA, Deoxycholic acid: DCA, Lithocholic acid: LCA and Ursodeoxycholic acid: UDCA) has been developed after preliminary extraction with XAD-2 resin. Enzymatic hydrolysis prevents the formation of interferring degradation products. Ether extraction with centrifugation eliminates water soluble interferring substances. Derivatization to hexafluorpropyl- instead of methyl esters results in better separation, shortens analysis time and prolongs the life span of the column. |
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| Keywords: | Gas chromatography Glass capillary columns Bile acids Hexafluoroisopropyl esters |
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