Kinetics of unfolding the human telomeric DNA quadruplex using a PNA trap |
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Authors: | Green Jeremy J Ying Liming Klenerman David Balasubramanian Shankar |
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Affiliation: | Department of Chemistry, University of Cambridge, Lensfield Road, UK. |
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Abstract: | The kinetics of opening of the DNA quadruplex formed by the human telomeric repeat have been investigated using real-time fluorescence resonance energy transfer (FRET) measurements with a peptide nucleic acid (PNA) trap. It has been found that this opening is zero-order with respect to PNA, indicating that the initial step is a rate-limiting internal rearrangement of the quadruplex. A study of the temperature dependence of the rate of quadruplex opening was performed and the activation energy of the process estimated to be 98 +/- 8 kJ mol(-1). |
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