高效液相色谱法同时测定血浆中的10种蛋白同化激素

建立了一种用于10种蛋白同化激素的同时分离检测的高效液相色谱法。根据被分析物的性质，以C18反相色谱柱为分离柱,以乙腈和水为流动相，采用梯度洗脱方式,并在194～290 nm的范围内快速调节检测波长,使各物质均在最大吸收波长处被检出。在优化的条件下,10种被测组分在10 min内实现了快速的基线分离,检出限在0.01～0.10 μg/mL范围内。在兔血浆中进行加标回收率测定,10种被测组分的加标回收率为70.3%～120%。选取美雄醇为代表进行实际动物实验,成功检测到耳脉注射美雄醇后兔血浆内的美雄醇成分。实验结果表明该方法可行，快速简便，准确可靠。

Simultaneous analysis of ten anabolic steroids in blood plasma using high performance liquid chromatography

A method for the simultaneous separation and determination of ten anabolic steroids in blood plasma using high performance liquid chromatography (HPLC) was established. An RP-C18 column was used as the analytical column, and the mixture of acetonitrile and water was used as the mobile phase with gradient elution according to the characteristics of the analytes. The analytes were detected at the adjustable wavelengths ranging from 194 to 290 nm. Under the optimal conditions, ten compounds were separated within 10 min. The detection limits were in the range of 0.01-0.10 μg/mL. The spiked recoveries of standards in a rabbit plasma sample were from 70.3%to 120%. Methandriol was injected into the ear meridian of a rabbit, and then the anabolic steroid methandriol in the plasma was successfully detected with the established method. The results show that the method is feasible, rapid, simple and accurate.