Multienzymatic Sucrose Conversion into Fructose and Gluconic Acid through Fed-Batch and Membrane-Continuous Processes |
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Authors: | Fadi Antoine Taraboulsi Jr Ester Junko Tomotani Michele Vitolo |
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Institution: | 1. University of S?o Paulo, School of Pharmaceutical Sciences, Av. Prof. Lineu Prestes, 580, B.16, 05508-900, S?o Paulo, SP, Brazil
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Abstract: | Multienzymatic conversion of sucrose into fructose and gluconic acid was studied through fed-batch and continuous (in a membrane reactor) processes. The law of substrate addition (sucrose or glucose) for the fed-batch process which led to a yield superior to 80% was the decreasing linear type, whose feeding rate (?; L/h) was calculated through the equation: ? = ?o ? k.t, where ?o (initial feeding rate, L/h), k (linear addition constant, L/h 2), and t (reaction time, h). In the continuous process, the yield of conversion of sucrose (Y) was superior to 70% under the following conditions: dilution rate?=?0.33 h?1, total duration of 15 h, pH 5.0, 37 °C and initial sucrose concentration of 64 g/L (Y?=?92%), 100 g/L (Y?=?83%), or 150 g/L (Y?=?76%). |
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