Norfloxacin La(III)-based complex: synthesis,characterization, and DNA-binding studies |
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| Authors: | Shaban Y. Shaban Maged A. El-Kemary Gehan Samir Hosny El-Baradei Ralph Puchta |
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| Affiliation: | 1. Faculty of Science, Department of Chemistry, Kafrelsheikh University, Kafrelsheikh, Egyptshaban.shaban@sci.kfs.edu.eg;3. Faculty of Science, Department of Chemistry, Kafrelsheikh University, Kafrelsheikh, Egypt;4. Inorganic Chemistry, Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany |
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| Abstract: | A La(III) complex, [LaIIICl2(NOR)2]Cl (2), containing norfloxacin (NOR) (1), a synthetic fluoroquinolone antibacterial agent, has been synthesized and characterized by elemental analysis, IR, UV–vis spectra and 1H NMR spectroscopy, and molar conductance measurements. The interaction between 2 and CT-DNA was investigated by steady-state absorption and fluorescence techniques in different pH media, and showed that 2 could bind to CT-DNA presumably via non-intercalative mode and the La(III) complex showed moderate ability to bind CT-DNA compared to other La(III) complexes. The binding site number n, and apparent binding constant KA, corresponding thermodynamic parameters ΔG#, ΔH#, ΔS# at different temperatures were calculated. The binding constant (KA) values are 0.23 ± 0.05, 0.56 ± 0.05, and 0.18 ± 0.08 × 105 L mol?1 for pH 4, 7, and 11, respectively. It was also found that the fluorescence quenching mechanism of CT-DNA by La(III) complex was a static quenching process. |
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| Keywords: | La(III) complexes Norfloxacin DNA-binding Fluorescence spectra Excitations |
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