| 摘 要： | For the study of invasion of tumor cells in vitro and in vivo methods were used to observe theinvasion process and morphological characteristics of tumor cells. The different animal tumor cells andcell lines from human tumors were used. The process of cancer cells in vitro invasion can be dividedinto 5 stages. We suggest that filopodia protruding from the tumor cell margin seem to play an im-portant role in the initiation of cell invasion. For experimental study of cancer metastasis we established three spontaneous metastatic models, in-cluding spontaneous lymphatic and hematogenous metastasis of mouse uterine cervix carcinoma No 14(U_(14))and No 27(U_(27)), and third spontaneous lung metastasis in mouse forestomach carcinoma (FC). Two tumorcell lines are established: One is MFC cell line which is derived from FC tumor; second cell line isMUCC which is derived from the U27 tumor. These metastasis models and their cell lines are veryuseful for studying the mechanism of metastasis, tumor heterogenicity an
|
