Zymomonas mobilis as catalyst for the biotechnological production of sorbitol and gluconic acid |
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Authors: | Gilmar Sidney Erzinger and Michele Vitolo |
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Affiliation: | (1) Pharmacy School, University of Joinville, Santa Catarina, SC, Brazil;(2) Biochemical and Pharmaceutical Technology Department Faculdade de Ciências Farmacêuticas, University of S?o Paulo, Av. Prof. Lineu Prestes, 580, B.16. 05508-900 S?o Paulo, SP, Brazil |
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Abstract: | The conversion of glucose and fructose into gluconic acid (GA) and sorbitol (SOR) was conducted in a batch reactor with free (CTAB-treated or not) or immobilized cells of Zymomonas mobilis. High yields (more than 90%) of gluconic acid and sorbitol were attained at initial substrate concentration of 600 g/L (glucose plus fructose at 1:1 ratio), using cells with glucose-fructose-oxidoreductase activity of 75 U/L. The concentration of the products varied hyperbolically with time according to the equations (GA)=t(GA)max/(WGA +t), (SOR)=t (SOR)max/(WSor+t), vGA=[WGA (GA)max]/(WGA+t)2 and VSOR=[WSOR (SOR)max]/(WSOR+t)2. Taking the test carried out with free CTAB-treated cells as an example, the constant parameters were (GA)max= 541 g/L, (SOR)max=552 g/L, WGA=4.8h, WSOR=4.9h, υGA=112.7 g/L· and υSOR=112.7 g/L·. |
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Keywords: | Zymomonas mobilis sorbitol gluconic acid |
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