Negative ion electrospray high‐resolution tandem mass spectrometry of polyphenols |
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Authors: | Jürgen Schmidt |
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Institution: | Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Halle/Saale, Germany |
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Abstract: | Representative compounds with a 1,3‐dihydroxybenzene substructure belonging to different important polyphenol classes (stilbenes, flavones, isoflavones, flavonols, flavanones, flavanols, phloroglucinols, anthraquinones and bisanthraquinones) were investigated based on detailed high‐resolution tandem mass spectrometry measurements with an Orbitrap system under negative ion electrospray conditions. The mass spectral behaviour of these compound classes was compared among each other not only with respect to previously described losses of CO, CH2CO and C3O2 but also concerning the loss of CO2 and successive specific fragmentations. Furthermore, some unusual fragmentations such as the loss of a methyl radical during mass spectral decomposition are discussed. The obtained results demonstrate both similarities and differences in their mass spectral fragmentation under MSn conditions, allowing a characterization of the corresponding compound type. Copyright © 2015 John Wiley & Sons, Ltd. |
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Keywords: | negative ion electrospray high‐resolution tandem mass spectrometry Orbitrap polyphenols stilbenes flavonoids anthraquinones detailed fragmentation studies combined CID/HCD experiments |
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