Development of an efficient and low-cost protocol for the manual PNA synthesis by Fmoc chemistry |
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| Authors: | Concetta Avitabile,Luca D. D&rsquo Andrea,Alessandra Romanelli |
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| Affiliation: | a University of Naples ‘Federico II’, School of Biotechnological Sciences, via Mezzocannone 16, 80134 Naples, Italy
b Institute of Biostructure and Bioimaging, CNR, via Mezzocannone 16, 80134 Naples, Italy |
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| Abstract: | An efficient and low-cost protocol for the manual synthesis of Peptide Nucleic Acids is reported here. The protocol relies on coupling reactions carried out with 2.5 equiv of PNA monomers activated with HOBT/HBTU, in the presence of pyridine/NMM. The protocol has been tested on four PNA oligomers with a length ranging from 9 to 12 bases and a purine content up to 67%. |
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| Keywords: | Peptide nucleic acid Manual synthesis Pyridine Fmoc |
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