Instantaneous, stoichiometric generation of powerfully reducing states of protein active sites using Eu(II) and polyaminocarboxylate ligands |
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Authors: | Vincent Kylie A Tilley Gareth J Quammie Nina C Streeter Ian Burgess Barbara K Cheesman Myles R Armstrong Fraser A |
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Institution: | Inorganic Chemistry Laboratory, Oxford University, South Parks Road, Oxford, UK OX1 3QR. |
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Abstract: | Addition of an equivalent of a polyaminocarboxylate ligand (L) to a solution of a redox protein and the aqua Eu2+ ion results in the instantaneous in situ generation of a very powerful reductant Eu(II)-L that can rapidly drive an electron stoichiometrically onto a redox centre having an extremely negative reduction potential (lower than -1 V): this is exemplified by straightforward generation of the super-reduced state of the Fe-protein of nitrogenase. |
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