Photoassisted Synthesis of Luminescent Mannose–Au Nanodots for the Detection of Thyroglobulin in Serum |
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| Authors: | Chih‐Ching Huang Prof Yu‐Lun Hung Yen‐Chun Shiang Tai‐Yuan Lin Prof Yu‐Shen Lin Chao‐Tsen Chen Prof Huan‐Tsung Chang Prof |
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| Institution: | 1. Institute of Bioscience and Biotechnology, Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University, 2, Beining Road, Keelung, 20224 (Taiwan), Fax: (+886)?2‐2462‐2320;2. Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 10617 (Taiwan), Fax: (+886)?2‐3366‐1171 (Huan‐Tsung Chang), Fax: (+886)?2‐2363‐6359 (Chao‐Tsen Chen);3. Institute of Optoelectronic Sciences, National Taiwan Ocean University |
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| Abstract: | We have employed mannose‐modified gold nanodots (Man–Au NDs) as a luminescence sensor for the detection of the thyroid‐cancer marker thyroglobulin (Tg) in homogeneous solutions. The luminescent Man–Au NDs are prepared through the reaction of 2.9 nm‐diameter gold nanoparticles (Au NPs) with 11‐mercapto‐3,6,9‐trioxaundecyl‐α‐D ‐mannopyranoside (Man‐RSH) under the irradiation of a light‐emitting diode (LED). We have found that the irradiation enhances the quantum yield (～11 %), alters the emission wavelength and lifetimes, and shortens the preparation time. A luminescence assay has been developed for Tg based on the competition between Tg and Man–Au NDs for the interaction with the concanavalin A (Con A). Because luminescence quenching of the Man–Au NDs by Con A is inhibited by Tg selectivity, we have obtained a highly sensitive and selective assay for Tg. |
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| Keywords: | cancer glycoproteins gold luminescence nanoparticles |
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