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饲喂两种中药配方对异育银鲫巨噬细胞免疫机能的影响
引用本文:袁春涛,孙方方,李冬梅,龚昳,吴光红,沈美芳,韩晓冬.饲喂两种中药配方对异育银鲫巨噬细胞免疫机能的影响[J].南京大学学报(自然科学版),2007,43(2):152-156.
作者姓名:袁春涛  孙方方  李冬梅  龚昳  吴光红  沈美芳  韩晓冬
作者单位:[1]南京大学医学院免疫与生殖实验室,南京210093 [2]江苏省淡水水产研究所,南京210017
基金项目:江苏省自然科学基金 , 江苏省水产三项工程
摘    要:配制3种分别含0.03%强普素、0.5%中药组方和1%中药组方的饲料,投喂异育银鲫30 d后分离纯化头肾巨噬细胞,用于测定巨噬细胞吞噬力和巨噬细胞呼吸氧爆发.结果显示,0.5%中药组异育银鲫头肾巨噬细胞吞噬力和呼吸氧爆发最强,其次为1%中药组,与对照组相比差异显著(P<0.05),而强普素组巨噬细胞吞噬力和呼吸氧爆发与对照组比无显著差异(P>0.05).研究表明,0.5%和1%中药组方能够显著增强异育银鲫头肾巨噬细胞的吞噬力和呼吸氧爆发强度.对提高异育银鲫的免疫机能有显著作用.

关 键 词:中药组方  强普素  异育银鲫  巨噬细胞吞噬力  呼吸氧爆发
修稿时间:09 1 2006 12:00AM

Effects of Two Different Traditional Chinese Medicine Formulae on Macrophage Function of Carassius auratus gibelio
Yuan Chun-Tao , Sun Fang-Fang , Li Dong-Mei , Gong Yi , Wu Guang-Hong , Shen Mei-Fang , Han Xiao-Dong.Effects of Two Different Traditional Chinese Medicine Formulae on Macrophage Function of Carassius auratus gibelio[J].Journal of Nanjing University: Nat Sci Ed,2007,43(2):152-156.
Authors:Yuan Chun-Tao  Sun Fang-Fang  Li Dong-Mei  Gong Yi  Wu Guang-Hong  Shen Mei-Fang  Han Xiao-Dong
Institution:1. Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing, 210093, China; 2. Freshwater Fisheries Research Institute, Nanjing, 210017, China
Abstract:Diets containing traditional Chinese medicine at 0.5%,1% and Qompsell at 0.03% were prepared to administrate Carassius auratus gibelio for 30 days.Macrophage from a head kidney was isolated for assaying macrophage phagocytic activity,macrophage reactive oxygen intermediates(ROIs).The results showed that the diets with 0.5% and 1% TCM resulted in significant increase in the macrophage phagocytic activity and macrophage ROIs(P<0.05)and 0.5%TCM was higher in macrophage phagocytic activity and macrophage ROIs than 1%TCM.But the 0.03% Qompsell diet had no significant difference in macrophage phagocytic activity and macrophage ROIs compared to control.These results indicated that the traditional Chinese medicine formula might elevate the function of macrophage especially the 0.5% TCM diet.And the 0.03% Qompsell diet had no effect on macrophage of Carassius auratus gibelio.
Keywords:traditional Chinese medicines formula  Qompsell  Carassius auratus gibelio  macrophage phagocyticactivity  reactive oxygen intermediates
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