Antimicrobial Agents Act Differently on Staphyloccocus aureus and Ralstonia eutropha Flavohemoglobins |
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Authors: | Aymen Ezzine Myriam Moussaoui Emna El Hammi Mohamed Nejib Marzouki Laura Baciou |
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Affiliation: | 1. Laboratoire de Chimie Physique, Université Paris Sud, UMR8000 CNRS, Faculté des Sciences, Bat. 350, 91405, Orsay, Cedex, France 2. Laboratoire d’ingénierie des protéines et molécules bioactives, Institut National des Sciences Appliquées et de Technologie, Université de Carthage, 1080, Tunis, Tunisia
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Abstract: | Flavohemoglobins (FlavoHb) play a key role in bacterial resistance to nitrosative stress and NO signaling modulation. In this study, we cloned, expressed, and characterized the flavoHb from the opportunistic pathogen, Staphylococcus aureus. The higher amino-acid sequence homology is shared with that from Saccharomyces cerevisiae which was therefore used to build a model structure by homology modeling. Interestingly, the high sequence homology with S. cerevisiae did not correlate with the enzymatic and kinetic properties which are much similar to those of Escherichia coli. In vitro and aerobically, we showed that S. aureus and Ralstonia eutropha flavoHbs accept cytochrome c and oxygen as substrates. Based on this feature, we investigated the preferences for both substrates depending on miconazole or econazole addition and found that the inhibitor chemical composition is determinant. |
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