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| 压电DNA传感器检测模式对频率漂移的影响 | |
| 引用本文： | 杨云,陈洪,聂立波,贺全国,何农跃.压电DNA传感器检测模式对频率漂移的影响[J].分析科学学报,2006,22(4):441-443. |
| 作者姓名： | 杨云 陈洪 聂立波 贺全国 何农跃 |
| 作者单位： | 1. 湖南工业大学纳米生物医药与器件实验室,湖南,株洲,412008 2. 中南林业科技大学生命科学技术系,长沙,410004 3. 东南大学分子电子学实验室,南京,210096 |
| 摘 要： | 利用分子自组装技术将DNA探针固定在石英金膜表面与生物素标记的靶DNA杂交,再结合经40 nm胶体金标记的亲核素,放大检测频率以提高检测灵敏度。靶DNA质量经胶体金放大后,QCM的频率降达到了86 Hz,其检出限可达10 fmol/L。在其检测过程中,分别考察了裸DNA晶片直接检测和晶片组装在底座中检测两种模式。前者由于杂交液扩散和溶液挥发,易造成频率检测不稳定和失真,后者由于避免了溶液扩散和挥发,能有效提高频率检测的稳定性和准确度。 |
| 关 键 词： | 压电DNA传感器 检测模式 频率漂移 |
| 文章编号： | 1006-6144(2006)04-0441-03 |
| 收稿时间： | 2005-06-30 |
| 修稿时间： | 2005-11-04 |
Influence of Measurement Modes on Frequency Drifts in Piezoelectricity DNA Detection |
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| YANG Yun,CHEN Hong,NIE Li-bo,HE Quan-Guo,HE Nong-yue.Influence of Measurement Modes on Frequency Drifts in Piezoelectricity DNA Detection[J].Journal of Analytical Science,2006,22(4):441-443. | |
| Authors: | YANG Yun CHEN Hong NIE Li-bo HE Quan-Guo HE Nong-yue |
| Institution: | 1. Laboratory of Nano-Biomedicines and Devices, Hunan University of Technology, Zhuzhou , Hunan 412008 ;2. Department of Life Science and Technology ,Central South University of Forestry and Technology ,Changsha 410004; 3. China Key Laboratory of Molecular and Biomolecular Electronics of Ministry of Education, Southeast University, Nanjing 210096 |
| Abstract: | HS-DNA probe was immobilized on the gold surface of quartz crystal oscillator by self-assembling.After hybridization,target DNA was connected with 40 nm Au nanoparticle via biotin-streptavidin conjugation to enlarge the frequency change and improve the sensitivity of detection.The frequency change reached to 86 Hz and the detection limit reached to 10 fmol/L in our experiments.Two measurement modes-naked crystal measurement and holder equipment mode were investigated.It was found that the frequency measurement was unstable and distorted under the former mode due to the diffusion of hybridization solution and the volatilization of buffer solution.However,this situation did not appear under the latter mode,which could ensure the stability and accuracy of the frequency measurement effectively. |
| Keywords: | Piezoelectricity DNA sensor Measurement mode Frequency drift |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |