Redox buffering of hypochlorous acid by thiocyanate in physiologic fluids |
| |
Authors: | Ashby Michael T Carlson Amy C Scott M Jared |
| |
Institution: | Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, Oklahoma 73019, USA. mashby@ou.edu |
| |
Abstract: | The major antimicrobial products of neutrophilic myeloperoxidase (MPO) in physiologic fluids are hypochlorous acid (HOCl) and hypothiocyanite (OSCN-), and the former is generally believed to be the killing agent. However, we have determined that HOCl oxidizes SCN- in a facile nonenzymic reaction. The observed kinetics and computational models substantiate the hypothesis that SCN- serves to moderate the potential autotoxicity of HOCl by restricting its lifetime in physiologic fluids. Furthermore, the oxidizing equivalents of HOCl are preserved in OSCN-, a more discriminate biocide that is not lethal to mammalian cells. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|