Simple reversed-phase liquid chromatography method for determination of tocopherols in edible plant oils |
| |
Authors: | Gliszczyńska-Swig?o Anna Sikorska Ewa |
| |
Institution: | Faculty of Commodity Science, Poznań University of Economics, al. Niepodleg?o?ci 10, 60-967 Poznań, Poland |
| |
Abstract: | A simple and rapid reversed-phase high-performance liquid chromatography method for determination of alpha-, (beta + gamma), and delta-tocopherols in edible plant oils has been developed. Oils are diluted in 2-propanol and injected directly onto Symmetry C18 column. Methanol and acetonitrile (1:1) are used as a mobile phase. Tocopherols are detected using fluorescence detector set at excitation and emission wavelength 295 nm and 325 nm, respectively. The method is precise (R.S.D. not higher than 2.24%) and sensitive-detection limits (DL) are 8 ng/ml for gamma- and delta-tocopherols and 28 ng/ml for alpha-tocopherol; quantification limits (QL) were calculated as three times higher than DL. |
| |
Keywords: | Tocopherols Oils Vitamins Food analysis |
本文献已被 ScienceDirect PubMed 等数据库收录! |