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Method for determination of microcystin-leucine-arginine in water samples based on the quenching of the fluorescence of bioconjugates between CdSe/CdS quantum dots and microcystin-leucine-arginine antibody |
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| Authors: | Xiaopan Zhou Yuanhua Meng Hongbing Ma Guanhong Tao |
| Institution: | 1. College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, Jiangsu, 215123, People’s Republic of China 2. College of Life Science, Soochow University, Suzhou, Jiangsu, 215123, People’s Republic of China |
| Abstract: | We have developed a method for the determination of microcystin-leucine-arginine (MC-LR) in water samples that is based on the quenching of the fluorescence of bioconjugates between CdSe/CdS quantum dots (QDs) and the respective antibody after binding of MC-LR. The core-shell CdSe/CdS QDs were modified with 2-mercaptoacetic acid to improve water solubility while their high quantum yields were preserved. Monoclonal MC-LR antibody was then covalently bioconjugated to the QDs. It was found that the fluorescence intensity of the bioconjugates was quenched in the presence of MC-LR. A linear relationship exists between the extent of quenching and the concentration of MC-LR. Parameters affecting the quenching were investigated and optimized. The limit of detection is 6.9?×?10?11 mol L?1 (3σ). The method was successfully applied to the determination of MC-LR in water samples. Bioconjugates of CdSe/CdS quantum dots and anti-microcystin-leucine-arginine (MC-LR) antibody were prepared through step A to C. Their fluorescence intensity was quenched linearly with addition of MC-LR at different concentrations (step D). A method for determination of MC-LR was thus established and it was simple, sensitive and specific with low-cost instrumentation |
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