|
|||||
|
|
LC–APCI–MS Quantitative Analysis of Curcumin in Rabbit Plasma |
|
| Authors: | Xuezhi Yang Jianshe Ma Yun Ye Yuan Zhang Guanyang Lin Yuancai Zheng Xu Li Xianqin Wang |
| Institution: | 1. The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000, China 2. School of Basic Medical Sciences of Wenzhou Medical College, Wenzhou, 325035, China 3. Analytical and Testing Center of Wenzhou Medical College, Wenzhou, 325035, China |
| Abstract: | A simple and specific liquid chromatography–atmospheric pressure chemical ionization–mass spectrometry (LC–APCI–MS) method was developed and validated for quantitative analysis of curcumin in rabbit plasma. Chromatographic separation was carried out on Zorbax SB-C18 column, acetonitrile-methanol–water used as mobile phase with gradient elution. APCI source was applied and operated in negative ion mode, and selective ion monitoring mode used to quantify curcumin. The method showed excellent intra-assay and inter-assay precision (relative standard deviations and bias <15%) for quality control (QC) samples. This method was successfully applied to a pharmacokinetic study of curcumin after intravenous administration to rabbits. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |