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The electrochemistry of uric acid at a gold electrode modified with L-cysteine, and its application to sensing uric urine |
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| Authors: | Yazhen Wang |
| Affiliation: | 1. School of Chemistry and Environmental Engineering, Jianghan University, Wuhan, 430056, China |
| Abstract: | The electrochemistry of uric acid at a gold electrode modified with a self-assembled film of L-cysteine was studied by cyclic voltammetry and differential pulse voltammetry. Compared to the bare gold electrode, uric acid showed better electrochemical response in that the anodic peak current is stronger and the peak potential is negatively shifted by about 100 mV. The effects of experimental conditions on the oxidation of uric acid were tested and a calibration plot was established. The differential pulse response to uric acid is linear in the concentration range from 1.0?×?10?6 to ~?1.0?×?10?4 mol?L?1 (r?=?0.9995) and from 1.0?×?10?4 to ~?5.0?×?10?4 mol?L?1 (r?=?0.9990), the detection limit being 1.0?×?10?7 mol?L?1 (at S/N?=?3). The high sensitivity and good selectivity of the electrode was demonstrated by its practical application to the determination of uric acid in urine samples. Cyclic voltammograms of UA at the bare electrode (a,b) and the L-Cys/Au electrode (c,d,e) in HAc-NaAc buffer containing different concentrations of UA. (a,c): blank; (b, d): 2.0?×?10?5 mol?L?1; (e) 4.0?×?10?5 mol?L?1. Scan rate: 100 mV?s?1 |
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