Lateral flow immunoassay for progesterone detection |
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| Authors: | V. A. Safronova J. V. Samsonova V. G. Grigorenko A. P. Osipov |
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| Affiliation: | 1. Division of Chemical Enzymology, Department of Chemistry, Moscow State University, Moscow, 119991, Russia
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| Abstract: | A new express method based on lateral flow immunoassay (LFIA) for progesterone detection was developed. To increase the assay sensitivity an enzyme label (horse-radish peroxidase) was used instead of colloidal gold. An optimal assay format was chosen and the influence of a range of buffer supplements (detergents, proteins and sucrose) was investigated by enzyme-linked immunosorbent assay (ELISA). Linear range of LFIA was between 2 and 40 ng/mL in buffer. Limit of detection was 2 ng/mL, assay time was within 15 min. |
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