Role of inorganic phosphate during electron transfer in the redox system NADH+flavin |
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| Authors: | T A Lozinova O N Brzhevskaya A V Kisin V M Nosova S V Rykov L Yu Tychinskaya |
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| Institution: | (1) N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow;(2) State Science Center of the Russian Federation/State Scientific-Research Institute of Heteroorganic Chemistry and Technology, Moscow;(3) Institute of Physical Organic Chemistry (State Scientific Institution), National Academy of Sciences of Belarus, 13 ul. Surganova, Minsk, 220072, Belarus |
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| Abstract: | We have used 31P NMR and EPR to study the effect of inorganic phosphate (Pi) on the process of electron transfer in oxidation of reduced nicotinamide adenine dinucleotide (NADH) by flavin in aqueous
solutions. Observation of a significant (up to 40%) drop in the integrated intensity of the 31P NMR signal for the inorganic phosphate over the course of the reaction is interpreted as a manifestation of the effect of
chemically induced polarization of the phosphorus nuclei during singlet-triplet conversion in charge-transfer complexes NADH…Pi], and is evidence for a possible catalytic role for Pi in processes involving transfer of an electron (hydrogen atom) from NADH to appropriate acceptors. The results presented
support the possibility of direct involvement of Pi in a chain of one-electron conversions in chemical systems.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 73, No. 3, pp. 392–396, May–June, 2006. |
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| Keywords: | electron paramagnetic resonance nuclear magnetic resonance chemically induced nuclear polarization flavin flavin mononucleotide inorganic phosphate adenosine-5′ -diphosphate adenosine 5′ -triphosphate nicotinamide adenine dinucleotide |
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